By American Academy of Ophthalmology, Robert H. Rosa Jr. MD
Part four provides fabrics in components: half I, Ophthalmic Pathology; and half II, Intraocular Tumors: scientific facets. half I makes use of a hierarchy that strikes from common to express to aid derive a differential prognosis for a selected tissue. half II is a compilation of chosen medical points of significance to the overall ophthalmologist. Following half II are the yankee Joint Committee on melanoma 2010 staging kinds for ocular and adnexal tumors.
Upon of entirety of part four, readers can be capable to:
Describe a dependent method of knowing significant ocular stipulations in accordance with a hierarchical framework of topography, illness procedure, basic analysis and differential diagnosis
Summarize the stairs in dealing with ocular specimens for pathologic examine, together with acquiring, dissecting, processing, and marking tissues
Identify these ophthalmic lesions that point out systemic affliction and are in all likelihood lifestyles threatening
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Additional info for 2014-2015 Basic and Clinical Science Course (BCSC): Section 4: Ophthalmic Pathology and Intraocular Tumors
Ately 10:1 for at least 24 hours prior to processing e 111 1onna eY· . dequate fixation. Differen institutions may use different protocols, and preop- to en ur ea ' . . e consultation 1s cntical. era t ,v ns >n. ild '0 ,rs as or Tissue Processing The infiltration and embedding process removes most of the water from the tissue and relaces the water with paraffin. Organic solvents used in this process will dissolve lipid and ~ay dissolve some synthetic materials. Routine processing usually dissolves intraocular lenses made of polymethylmetbacrylate (PMMA), polypropylene, and silicone, although the PMMA may fall out during sectioning.
Clinically used transc riptaseto determine the polymerase chai n expression of a gene reaction (AT-PCRl 0 Real-time quantitative PCR (RT-PCR) Measurement of PCR-product accumulation during the exponential phase of the PCR reaction using a dual-labeled fluorogenic probe Comparative genomic hybrid ization (CGH) Molecular cytog enetic method for analysis of copy number changes (gains/ losses) in the DNA content of an individual, often in tumor cells. Uses epifluorescence and quantitative, regional differences in the fluorescence ratio of gains/ losses vs control DNA to identify abnormal regions in the genome at a resolution of 20-80 base pairs Differentially labeled test and reference DNAs, hybridized to cloned fragments, genomic DNA or cDNA, which are spotted on a glass slide (the array).
This anatomical arrangement is the basis of the evisceration technique and explains the vulnerability of the eye to expulsive choroi- A break in the Descemet membrane in keratoconus shows anterior curling of Descemet membrane toward the corneal stroma (arrow). (Courtesy of Hans E. Grossniklaus. / Figure 2-3 A break in the Descemet tnembrane as a result of forc::eps injury shows anterior curling of the original membrane (arrow) and production of a secondary thickened membrane. (Couriesy of Hans E.