Analysis of Genetic Variation in Animals by M. Caliskan

By M. Caliskan

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Microsatellites are useful markers for population genetic studies because they offer advantages which are particularly appropriate for conservation projects: they are widely available and exhibit a high degree of polymorphism. In addition, it is assumed they are neutral to selection, since the observed genetic diversity is constituted as the consequence of two forces: genetic drift and mutation. Worldwide, there is a special concern regarding the evaluation and genetic characterization of local populations, the so-called rare animal breeds.

Troy, C. S. et al. 2001. Genetic evidence for Near-Eastern origins of European cattle. Nature 410: 1088-1091. Vicente, A. A. et al. 2008. Genetic diversity in native and commercial breeds of pigs in Portugal assessed by microsatellites. Journal of animal science 86: 2496. Weber, J. L. 1990. Human DNA polymorphisms based on length variations in simplesequence tandem repeats. Genome analysis 1: 159-181. Weir, B. ; and C. C. Cockerham. 1984. Estimating F-statistics for the analysis of population structure.

2009). The combined PCR products were detected by capillary electrophoresis using an ABI Prism 310 DNA Genetic Analyzer (AppliedBiosystems) and the size of alleles was determined using GeneScan-500 LIZ Size Standard (AppliedBiosystems). A total of 112 different alleles were identified for 10 microsatellites in all seven breeds analyzed (Table 7). The most polymorphic marker was SO005 with a total of 21 alleles, while microsatellite SO101 was the least polymorphic, showing only 7 alleles (Manea et al, 2009).

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